Figure 5

Confocal laser scanning microscopy images of machined titanium (a,c) and anodized titanium (b,d) surfaces incubated with a co-culture of A. oris and S. mitis. The bacteria are labeled with LIVE/DEAD® (Molecular Probes) thus green color indicates live cells and red color indicates dead cells. Surfaces a-c were incubated anaerobic and d aerobic. The two bacteria were found in equal amounts on the machined titanium surface after anaerobic incubation at pH 7 (a) while mostly A. oris could be found on the anodized surface, after incubation at the same conditions (b). The adhesion of S. mitis to both surfaces was further reduced after incubation at pH 5 irrespective of atmosphere (c,d). The number of non-viable cells (red) of A.oris was found to be greater after aerobic incubation than anaerobic (d).