Figure 5
From: Surface-associated MUC5B mucins promote protease activity in Lactobacillus fermentum biofilms
Surface proteins identified in biofilm cells of L. fermentum. (a) Western blotting of two-dimensional electrophoresis (2DE) of surface proteins. Samples were subjected to IEF in a pH 4–7 gradient followed by SDS-PAGE in 14% gels. After electroblotting onto PVDF membranes, gpr, O-sialoglycoprotein endopeptidase was detected using the polyclonal antiserum MOB-LFGE-2. (b) Gel stained with Coomassie Brilliant Blue after 2DE of surface proteins. Spots were excised from the stained gel, subjected to in-gel tryptic digestion and proteins identified by LC-MS/MS analysis. The proteins identified were: arg, arginine deiminase; ATPase, ATP synthase pyruvate dehydrogenase; DnaK; Ef-G, elongation factor G; Ef-Tu, elongation factor Tu; fum, fumarate hydratase; GroEl; ldh, lactate dehydrogenase; ket, phosphoketolase; orn, ornithine carbamoyltransferase; pdh, pyruvate dehydrogenase and pph, phophopyruvate hydratase.