Figure 3

CHD-FA is non-toxic against an oral epithelial cell line. An orally relevant epithelial cell line (OKF6/TERT2) was grown to 90% confluence in 24 well plates for toxicity studies. Cells were treated with 0.5% (v/v) CHD-FA at pH 2.0 and 7.0 and with 0.2% CHX for 30 min. After treatment, cells were carefully washed with PBS and cellular viability assessed using the alamarBlue® assay, with absorbance read at 570 and 600 nm. All samples were assayed in triplicate, on three independent occasions. Data represents mean ± SD (***p <0.0001).