Table 2 Sequences of primers and probes used in the quantitative PCR assays
From: Subgingival periodontal pathogens associated with chronic periodontitis in Yemenis
Test species | Sequences 5′-3′ | Target gene | Product size | Ref |
|---|---|---|---|---|
A. actinomycetemcomitans | F-primer: GGRAGAATGGATGGCGATAT | hgpA | 81 bp | This study |
R-primer: ATCAGAATGAACATAACCTATACCA | ||||
Probe: FAM- ATGAACGCAATTCAGCCCAGA ACTG-BHQ | ||||
P. micra | F-primer: TGAGCAACCTACCTTACACAG | 16S rRNA | 112 bp | [17] |
R-primer: GCCCTTCTTACACCGATAAATC | ||||
Probe: FAM- ACCGCATGAGACCACAGAA TCGCA-BHQ | ||||
P. gingivalis | F-primer: ACGAATCAAAGGTGGCTAAGTT | fimA | 85 bp | [17] |
R-primer: TTAGTCGCATTTTCGGCTGAT | ||||
Probe: FAM- CCTGCTGTTCTCCATTATAAAC CATTACGG -BHQ | ||||
T. forsythia | F-primer: GATAGGCTTAACACATGCAAGTC | 16S rRNA | 99 bp | [17] |
R-primer: GTTGCGGGCAGGTTACATAC | ||||
Probe: FAM- TTACTCACCCGTGCGCCGGTCG-BHQ | ||||
T. denticola | F-primer: GGGCGGCTTGAAATAATRATG | 16S rRNA | 92 bp | [17] |
R-primer: CTCCCTTACCGTTCGACTTG | ||||
Probe: FAM- CAGCGTTCGTTCTGAGCCA GGATCA-BHQ | ||||
Total bacteria | F-primer: AAACTCAAAGGAATTGACGGGG | 16S rRNA | 205 bp | [17] |
R-primer: TTGCGCTCGTTGCGGGACT | ||||
Probe: FAM-CTGTCGTCAGCTCGTGTCGTGA-BHQ | ||||
Fusobacterium spp.* | F-primer: CGCAGAAGGTGAAAGTCCTGTAT | 23S r RNA | 101 bp | [18] |
R-primer: TGGTCCTCACTGATTCACACAGA | ||||
Probe: FAM- CTTTGCTCCCAAGTAACATG GAACACGA-BHQ | ||||
Prevotella spp.* | F-primer: ACCAGCCAAGTAGCGTGCA | 16S rRNA | 153 bp | [19] |
R-primer: TGGACCTTCCGTATTACCGC | ||||
Probe: FAM- AATAAGGACCGGCTAATTCC GTGCCAG -BHQ |