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Table 1 Human primers used in RT-PCR for Glyceraldehyde-3 phosphate-dehydrogenase (GAPDH), alkaline phosphatase (ALP), A2 pro-collagen type 1 chain (COLL-1A2), bone morphogenetic protein-2,-5,-7 (BMP-2,-5,-7)

From: Analysis of human alveolar osteoblast behavior on a nano-hydroxyapatite substrate: an in vitro study

Target gene Primer sequences
GADPH 5′-GGAGTCAACGGATTTGGTCGTA-3′
5′-GGCAACAATATCCACTTTACCAGAGT-3′
ALP 5′-TGCGGAAGAACCCCAAAG-3′
5′-ATGGTGCCCGTGGTCAAT-3′
Coll-1A2 5′-CCCAGCCAAGAACTGGTATAGG-3′
5′-GGCTGCCAGCATTGATAGTTTC-3′
Osteocalcin 5′-AGCAAAGGTGCAGCCTTTGT-3′
5′-GCGCCTGGGTCTCTTCACT-3′
Osteonectin 5′-CCACACGTTTCTTTGAGACC-3′
5′-GATGTCCTGCTCCTTGATGC-3′
BMP-2 FW5′-CCAGCTGTAAGAGACACCCTTTG-3′
RV5′-ACCCACAATCCAGTCATTCCA-3′
BMP-5 FW5′-CGTCCTCTGCACCTCTCTTTATG-3′
RV5′-CTCCGACTCTTCAGGATTTTCTTC-3′
BMP-7 FW5′-TCTTCCACCCACGTACCA-3′
  RV5′-GCTTCCCCTTCTGGGATCTT-3′