Figure 4

Observation of JE and OGE cell morphology (primary cells). A. JE cell clones formed (the arrow × 200); B. At 10—12 d, JE cells presented non-uniform morphology and scattered arrangement (× 200); C. The 3rd passage JE cells multiple ‘giant cells’ appeared (the arrow × 200); D. The 5th passage JE cells showed signs of aging and death (× 400); E. the OGE cell clones formed and expanded (the arrow × 200); F. At 7—9 d, the OGE cells presented uniform morphology, tight arrangement, and ‘paving stone-like’ keratinizing (× 200); G. In the 3rd passage OGE cells ‘giant cells’ appeared (× 200); H. The 7th passage OGE cells showed signs of aging and death (× 400).