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Figure 1 | BMC Oral Health

Figure 1

From: Enamel matrix derivative protein enhances production of matrixmetalloproteinase-2 by osteoblasts

Figure 1

EMD protein enhances MG-63 cells degradation of gelatin. MG-63 cells were incubated in the presence (D, E, F) or absence (A, B, C) of 100 μg/ml EMD for 20 h, and were then incubated on glass coated with 100 μg/ml quenched fluorescent substrate DQ-gelatin for an additional 4 h. TIMP-2 (20 μM) was incubated with EMD protein and MG-63 cells as described above (G, H, I). Degradation of gelatin (green fluorescence) was detected by a confocal microscope (Excitation: 488 nm; Emission: 530 nm). Images were obtained at ×40 magnification (A through I). Differential interference contrast (DIC) images are shown. Quantification of GFP in Panels A-I was performed densitometrically using NIH image J software. Density is depicted as mean GFP per cell.

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