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Figure 2 | BMC Oral Health

Figure 2

From: Enamel matrix derivative protein enhances production of matrixmetalloproteinase-2 by osteoblasts

Figure 2

Expression of MMP-2 from EMD protein-stimulated MG-63 cells. (A) MG-63 cells were incubated in serum-free Eagle containing 100 μg/ml EMD protein for 24 h and conditioned media and total cell lysates were prepared as described in Methods. Concentrated media were separated by 8% SDS-PAGE, blotted with anti-MMP-2 antibody and visualized with Super Signal west pico chemiluminescent substrate. Molecular markers (kDa) are shown in the left column. Quantification of MMP-2 in the top panel was performed densitometrically using NIH image J software. Peak heights of each density are depicted as percent of maximum valuel. (B) Total RNA was isolated from MG-63 cells cultured in the presence of EMD for 24 h and subjected to RT-PCR experiments by using specific primers for MMP-2 (top) or GAPDH (bottom).

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