Figure 2
From: Role of extracytoplasmic function sigma factors in biofilm formation of Porphyromonas gingivalis
Biofilm formation by homotypicP. gingivalis33277 or ECF sigma factor mutants. The strains were grown on enriched BHI broth anaerobically at 37°C in collagen type-I-coated 12-well flat-bottom microplates. After 48 h of cultivation, the organized biofilm mass was evaluated by staining with crystal violet. (a) The photographs are a representative sample of each experimental strain. (b) Biofilm formation determined by crystal violet staining and adjusted for growth (A600 units per OD660 unit). The data shown are mean ± SD of triplicate experiments. ***, p < 0.001, by a one-way ANOVA Test/Dunnett’s Multiple Comparison Test.