Table 1 Bacterial strains and plasmids used in this study

E. coli strain

DH5α

General-purpose host strain for cloning

Invitrogen

P. gingivalis strain

33277

wild type

ATCC

KDP314

PGN_0274::ermF ermAM, Em^r

This study

KDP315

PGN_0319::ermF ermAM, Em^r

This study

KDP316

PGN_0450::ermF ermAM, Em^r

This study

KDP317

PGN_0970::ermF ermAM, Em^r

This study

KDP319

PGN_1740::ermF ermAM, Em^r

This study

KDP314C

KDP314/pKD828, Em^r Tc^r

This study

KDP319C

KDP319/pKD829, Em^r Tc^r

This study

E. coli plasmid

pGEM-T Easy

Ap^r, plasmid vector for TA cloning

Promega

pKD355

Ap^r, contains the ermF ermAM DNA cassette between EcoRI and BamHI of pUC18

12

pKD814

Ap^r, contains the 1.0-kb PCR-amplified fragment (PGN_0450 region) in pGEM-T Easy

This study

pKD817

Ap^r, contains the 1.5-kb PCR-amplified fragment (PGN_0274 region) in pGEM-T Easy

This study

pKD818

Ap^r, contains the 2.0-kb PCR-amplified fragment (PGN_0319 region) in pGEM-T Easy

This study

pKD819

Ap^r, contains the 2.0-kb PCR-amplified fragment (PGN_0970 region) in pGEM-T Easy

This study

pKD821

Ap^r, contains the 2.0-kb PCR-amplified fragment (PGN_1740 region) in pGEM-T Easy

This study

pKD822

Ap^r Em^r, contains the ermF ermAM DNA cassette at BamHI site within PGN_0274 of pKD817

This study

pKD823

Ap^r Em^r, contains the ermF ermAM DNA cassette at BamHI site within PGN_0319 of pKD818

This study

pKD824

Ap^r Em^r, contains the ermF ermAM DNA cassette at BamHI site within PGN_0450 of pKD814

This study

pKD825

Ap^r Em^r, contains the ermF ermAM DNA cassette at BglII site within PGN_0970 of pKD819

This study

pKD827

Ap^r Em^r, contains the ermF ermAM DNA cassette at BamHI site within PGN_1740 of pKD821

This study

P. gingivalis plasmid

pT-COW

Ap^r Tc^r, E. coli-P. gingivalis shuttle plasmid

14

pKD828

Ap^r Tc^r, pT-COW- PGN_0274⁺

This study

pKD829

Ap^r Tc^r, pT-COW- PGN_1740⁺

This study