Figure 4

Association of constitutive antimicrobial gene expression with the DEFB1 -44 SNP genotype. cDNA was made from primary keratinocytes from 12 donors grown in 0.15 mM Ca²⁺ (left) or 0.6 mM Ca²⁺ (right). QPCR amplification of cDNA for hBD1(A) and hBD3 (B) and the housekeeping gene RPO was performed. All data were normalized to the housekeeping gene and compared to a separate reference cDNA. The data presented are log2 transformed and represent 3 replicates for each cell line with duplicate QPCR reactions. The mean and std. dev. for each group is shown. *, p < 0.05; ** p < 0.01.