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Table 5 PCR results showing inhibition in S. noxia samples

From: Development of a polymerase chain reaction assay for the rapid detection of the oral pathogenic bacterium, Selenomonas noxia

 

PCR results (Ct values)

Sample

Dilution

Sample

IPC

S. noxia (ATCC 43541)

 
 

100

14.60

15.01

40.00

40.00

 

10−1

17.76

17.94

40.00

40.00

 

10−2

21.30

21.23

40.00

40.00

 

10−3

24.79

24.71

40.00

31.21

 

10−4

28.13

28.01

28.08

28.11

S. noxia (ATCC 51893)

 
 

100

13.36

13.24

40.00

40.00

 

10−1

15.34

15.45

40.00

40.00

 

10−2

19.59

19.26

40.00

40.00

 

10−3

22.54

23.05

40.00

40.00

 

10−4

28.29

28.23

40.00

40.00

 

10−5

30.80

30.77

28.08

28.93

S. noxia (ATCC 700225)

 
 

100

20.90

20.00

40.00

40.00

 

10−1

nd1

nd1

nd1

nd1

 

10−2

22.92

23.00

40.00

40.00

 

10−3

26.68

26.48

40.00

40.00

 

10−4

29.95

30.11

28.53

28.22

 

10−5

33.53

33.53

27.73

28.06

No template control

 

40.00

40.00

28.40

28.12

  1. 1not determined