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Table 6 Data showing dilution at which inhibition was resolved (n = 2 for all samples, except those in bold which consisted of four replicates)

From: Development of a polymerase chain reaction assay for the rapid detection of the oral pathogenic bacterium, Selenomonas noxia

  

PCR results (Mean Ct value)

Sample

Dilution

IPC

Bacillus cereus

1.00E + 00

40.00

1.00E-02

40.00

1.00E-04

40.00

1.00E-05

31.43

1.00E-06

29.48

Candida albicans

1.00E + 00

40.00

1.00E-01

30.76

Centipeda periodontii

1.00E + 00

40.00

1.00E-02

40.00

1.00E-03

40.00

1.00E-04

29.01

Klebsiella pneumonia

1.00E + 00

40.00

1.00E-01

30.42

Lactobacillus acidophilus

1.00E + 00

40.00

1.00E-01

40.00

1.00E-02

40.00

1.00E-03

29.84

Pseudomonas aeruginosa

1.00E + 00

40.00

1.00E-01

30.47

Pectinatus cerevisiiphilus

1.00E + 00

40.00

1.00E-01

40.00

1.00E-02

40.00

1.00E-03

28.70

Staphylococcus aureus

1.00E + 00

40.00

1.00E-02

40.00

1.00E-03

29.73

Selenomonas artemidis

1.00E + 00

40.00

1.00E-01

40.00

1.00E-02

40.00

1.00E-03

28.72

Selenomonas dianae

1.00E + 00

40.00

1.00E-01

40.00

1.00E-02

40.00

1.00E-03

28.22

Selenomonas flueggeii

1.00E + 00

40.00

1.00E-01

40.00

1.00E-02

40.00

1.00E-03

28.82

Selenomonas infelix

1.00E + 00

40.00

1.00E-01

40.00

1.00E-02

29.32

Streptococcus mutans

1.00E + 00

40.00

1.00E-01

40.00

1.00E-02

29.98

1.00E-03

28.25

Selenomonas sputigena

1.00E + 00

40.00

1.00E-01

40.00

1.00E-02

40.00

 

1.00E-03

28.56

Positive control

28.64

Negative control

29.23

No amplification control

40.00

  1. Bold font represents those samples consisting of four replicates