Fig. 4From: Salivary pellets induce a pro-inflammatory response involving the TLR4–NF-kB pathway in gingival fibroblastsPrior phase contrast and live-dead staining microscopy gingival fibroblasts were exposed to a series of dilutions of salivary pellet and pellet supernatant, 1, 2, 4, 8 fold, respectively, for 6 h. Visual inspection using phase contrast microscopy revealed a regular fibroblastic morphology. Live-dead staining showed that most of the cells were colored green, indicating that they were vital (10 fold magnification)Back to article page