Fig. 2

Hypoxia inducible factor-1 signaling is involved in the increase of angiogenin / ANGIOGENIN upon stimulation with L-mimosine or hypoxia. Dental pulp-derived cells (DPC) in monolayer cultures were treated with L-mimosine (L-MIM) at 1 mM or hypoxia with and without echinomycin at 1 μM to for 24 h. mRNA levels of angiogenin (a, b) and protein levels of ANGIOGENIN (c, d) were assessed by RT-qPCR and ELISA, respectively. a, b Bars represent mRNA levels as mean + standard deviation, relative to the normoxic control (dashed line). Experiments were conducted twice with three different donors, respectively. Kruskal Wallis test p < 0.05. post hoc Mann-Whitney * p < 0.05 c, d Bars represent mean + standard deviation of ANGIOGENIN relative to the normoxic control (dashed line). Experiments were conducted twice with 3 different donors, respectively. Kruskal Wallis test p < 0.05. post hoc Mann-Whitney * p < 0.05