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Fig. 4 | BMC Oral Health

Fig. 4

From: Effects of platelet rich plasma (PRP) on human gingival fibroblast, osteoblast and periodontal ligament cell behaviour

Fig. 4

Effect of PRP on human periodontal ligament cells. Human PDL cells cultured with PRP on (a) cell migration at 24 h, (b) cell proliferation at 1, 3 and 5 days, (c) real-time PCR at 3 and 14 days for mRNA levels of osteoblast differentiation markers including Runx2, COL1a2, ALP and OCN, (d) immunofluorescent COL1 staining at 14 days, (e, f) ALP staining both with and without osteoblast differentiation media (ODM) at 14 days, as well as (f) alizarin red staining denoting mineralization at 14 days post seeding. (Data presents means and standard error bars; * denotes significant difference between groups, p < 0.05)

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BMC Oral Health

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