Fig. 3

Sclerostin (SOST), dickkopf (DKK)-1, stromal cell-derived factor (SDF)-1, vascular endothelial growth factor (VEGF) and interleukin (IL)-8 upon exposure to L-mimosine (L-MIM) or hypoxia in monolayer cultures. Dental pulp-derived cells were cultured in 2D monolayer culture and treated with 1 mM L-MIM or hypoxia for 24 h. Changes in mRNA levels (Sost, Dkk-1, Sdf-1, Vegf, and Il-8) were analysed by qPCR (a, b, c, d, e) and changes in protein levels (SOST, DKK-1, SDF-1, VEGF, and IL-8) were analysed by ELISA (f, g, h, i, j). Data are displayed as mean ± standard deviation (black bars) and relative to the normoxic control (white bar). The level of significance was set at p < 0.05 (*). N equalled 6 or higher