Fig. 1
From: Molecular and clinical analyses of Helicobacter pylori colonization in inflamed dental pulp
Nested PCR method for H. pylori detection. a A schematic diagram of positions of the designed primers in this study. b Representative images showing the single PCR assay for H. pylori detection using ureA-aF and ureA-bR primer sets. c Representative images showing the nested PCR assay for H. pylori detection using ureA-aF and ureA-bR primer sets, followed by ureA-bF and ureA-aR primer sets. Lanes: 1, H. pylori 26,695; 2, J99; 3, ATCC 51932; 4, H. pullorum ATCC 51802; 5, H. felis ATCC 49179; 6, sterile water. M, molecular size marker (100 bp DNA ladder)