Rapid urease test (RUT) for evaluation of urease activity in oral bacteria in vitro and in supragingival dental plaque ex vivo

Table 1 Bacterial species tested for urease activity with the RUT test, read after 1 h incubation at 36 °C

Bacterial species	Origin or strain designation^a	Urease activity^b
Actinomyces naeslundii	OMGS 2466	+
Actinomyces naeslundii	OMGS 1923	0
Actinomyces oris	OMGS 2683	+
Campylobacter gracilis	CCUG 27720	+
Campylobacter rectus	OMGS 1236	+
Campylobacter ureolyticus	CCUG 7319	+++
Enterococcus faecalis	ATCC19433	0
Escherichia coli	OMGS 3935	0
Fusobacterium nucleatum	OMGS 2685	0
Haemophilus parainfluenzae	CCUG 12836 T	0
Haemophilus. parainfluenzae	OMGS 199/11	++
Haemophilus parainfluenzae	OMGS 202/11	+++
Haemophilus parainfluenzae	OMGS 203/11	+++
Helicobacter pylori	ATCC 43504	+++
Lactobacillus casei/paracasei	OMGS 3184	0
Lactobacillus. salivarius	CCUG 55845	0
Lactobacillus fermentum	OMGS 3182	+
Porphyromonas gingivalis	OMGS 2860	0
Prevotella intermedia	OMGS 2514	0
Pseudomonas aeruginosa	OMGS 3943	0
Rothia dentocariosa	OMGS 1956	0
Streptococcus mitis	CCUG 31611	+
Streptococcus mutans	OMGS 2482	0
Streptococcus salivarius I	OMGS 3944	0
Streptococcus salivarius II	OMGS 3945	+
Streptococcus sanguinis	OMGS 2478	0
Staphylococcus aureus	OMGS 3947	+
Staphylococcus epidermidis	OMGS 3949	+++
Tannerella forsythia	ATCC43037	0

^aATCC and CCUG means reference strains, OMGS means clinical isolates from the department collection
^bThe visual color change was graded as no reaction (0) for no color change, + denoted a weak reaction with a shift to pink color, ++ denoted a moderate reaction with a shift towards red and +++ a strong reaction with a clear purple color

ISSN: 1472-6831