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Fig. 3 | BMC Oral Health

Fig. 3

From: Oral lichen-planus-associated fibroblasts acquire myofibroblast characteristics and secrete pro-inflammatory cytokines in response to Porphyromonas gingivalis lipopolysaccharide stimulation

Fig. 3

OLP mucosal stromal cells in primary culture coexpress α-SMA and vimentin (a and b). Primary cultured cells from normal mucosa lacked α-SMA expression but expressed vimentin (c and d). Expression of α-SMA and vimentin mRNA harvested from AF and NF was evaluated by quantitative RT-PCR. mRNA expression of α-SMA from AF was significantly higher than that of NF. Vimentin expression showed no significant difference between the groups (p = 0.471) (e). Western blot analysis performed with total cellular proteins from NF and AF clones. AF expressed high levels of α-SMA, whereas fibroblast clones lacked expression. NF and AF showed no significant difference in vimentin expression. GAPDH was used as an internal control (f)

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