Fig. 2

Overexpression of WIF1 enhanced osteo/dentinogenic differentiation in SCAPs. (a, b) Real-time RT-PCR result (a) and Western blot result (b) showing that WIF1 was overexpressed in SCAPs due to retrovirus infection. GAPDH was used as an internal control. (c) ALP activity assays showing that ALP activity was enhanced in SCAPs that overexpressed WIF1 5 days after mineralization induction. (d, e) Alizarin Red staining (d) and calcium quantitative analysis (e) showing that overexpression of WIF1 enhanced mineralization in SCAPs 2 weeks after mineralization induction. Student’s t-test was performed to determine the statistical significance. All error bars represent the s.d. (n = 3). *P ≤ 0.05; **P ≤ 0.01