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Fig. 1 | BMC Oral Health

Fig. 1

From: Cyclic compression emerged dual effects on the osteogenic and osteoclastic status of LPS-induced inflammatory human periodontal ligament cells according to loading force

Fig. 1

Cell characterization and LPS-induced inflammation of hPDLCs in vitro. Primary cells grew out from the tissue explants (a) and were spindle shaped (b). Immunocytochemistry staining showed that cells were vimentin positive (c) and cytokeratin negative (d) (magnification: 100×, scale bar: 150 μm). After treated by different concentrations of LPS (range = 0–500 μg/ml) for 24, 48 and 72 h respectively, cell viability of hPDLCs was evaluated with MTT assay (e). mRNA expressions of pro-inflammatory cytokines IL-1β, IL-6, IL-8, MCP-1 and TNF-α in hPDLCs after 0.1, 1.0 and 10 μg/ml LPS treatment were detected using real-time PCR (f). Data were represented as means ± SEM, n = 6 (hPDLCs from six donors). The bars with different lowercase letters were significantly different from each other (P < 0.05), and those with the same letter exhibited no significant difference

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BMC Oral Health

ISSN: 1472-6831

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