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Fig. 4 | BMC Oral Health

Fig. 4

From: Cyclic compression emerged dual effects on the osteogenic and osteoclastic status of LPS-induced inflammatory human periodontal ligament cells according to loading force

Fig. 4

Expression of mRNA and protein levels of the pro-osteoclastic markers in hPDLCs after different dynamic cyclic stress loading for 5 days. a Real-time PCR results of pro-osteoclastic markers RANKL, M-CSF, CTSK and PTHLH mRNA expression in hPDLCs after different cyclic stress loading for 5 days with LPS or not. b Western blotting analysis for RANKL using total protein isolated from different groups of hPDLCs. c Quantification of Western blotting analysis. Protein content was expressed relative to the control and represented three similar independent experiments with triplicate observations in each experiment. Data were represented as means ± SEM, n = 6 (hPDLCs from six donors). The bars with different lowercase letters were significantly different from each other (P < 0.05), and those with the same letter exhibited no significant difference

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