Table 1 Primers used for quantitative real-time PCR analysis
Gene | Forward primer | Reverse primer |
|---|---|---|
IL-1β | AACCTCTTCGAGGCACAAGG | AGATTCGTAGCTGGATGCCG |
IL-6 | AGTGAGGAACAAGCCAGAGC | AGCTGCGCAGAATGAGATGA |
IL-8 | ACTGAGAGTGATTGAGAGTGGAC | AACCCTCTGCACCCAGTTTTC |
TNF-α | CTGCACTTTGGAGTGATCGG | TCTCTCAGCTCCACGCCATT |
MCP-1 | CAGCCAGATGCAATCAATGCC | TGGAATCCTGAACCCACTTCT |
ALP | GGACCATTCCCACGTCTTCAC | CCTTGTAGCCAGGCCCATTG |
COL-1 | CCAGAAGAACTGGTACATCAGCAA | CGCCATACTCGAACTGGAATC |
RUNX2 | CCCGTGGCCTTCAAGGT | CGTTACCCGCCATGACAGTA |
OCN | AATCCGGACTGTGACGAGTTG | CAGCAGAGCGACACCCTAGAC |
OPN | GCCGAGGTGATAGTGTGGTT | ACTCCTCGCTTTCCATGTGT |
OSX | CCTCTGCGGGACTCAACAAC | AGCCCATTAGTGCTTGTAAAGG |
RANKL | GAAAGCAAATGGTGTGGCCG | ACGTCACATCCCTGGTACAC |
M-CSF | CTA AGCTGGACGCACAGACCA | TCTCAGGCTGCACACCTT |
PTHLH | GTGTCCCCTAACTCCAAGCC | TTGAGCGGCTGCTCTTTGTA |
CTSK | GGGGGACATGACCAGTGAAG | CAGAGTCTGGGGCTCTACCT |
β-actin | TGGCACCCAGCACAATGAA | CTAAGTCATAGTCCGCCTAGAAGCA |