Fig. 1

a Results of water-soluble tetrazolium salt-1 assay for assessing the viabilities of hDPCs treated with PTHrP at different concentrations (1, 10, and 100 nM). The effects of PTHrP at 1 and 10 nM on mRNA expression levels of odontogenic markers were determined by quantitative reverse transcription-polymerase chain reaction. The expression levels of b DSPP and c DMP-1 in hDPCs stimulated with PTHrP at 1 or 10 nM over different time periods were determined. d DSPP and DMP-1 protein levels were increased after treatment with 1 nM or 10 nM of PTHrP for three and five days compared to those in the control without PTHrP treatment. (E-F) Graphic representation of DMP-1 and DSPP protein levels. These graphs were normalized to data from cells not treated with PTHrP. ^*Statistically significant difference compared to the no PTHrP group (P < 0.05), ^**Statistically significant difference compared to the no PTHrP group (P < 0.01). ^***Statistically significant difference compared to the no PTHrP group (P < 0.001)