Fig. 2

Effect of PTHrP on mineralization in hDPCs. (a) Cells cultured with or without PTHrP at 1 or 10 nM for seven days. ALP activity was evaluated by ALP staining. (b) Quantification of ALP staining. ALP staining was increased after PTHrP treatment for seven days compared to the GM control without PTHrP treatment. (c) Cells cultured with or without PTHrP at 1 or 10 nM for 14 days. Calcium nodule deposition was evaluated by Alizarin red S staining. (d) Quantification of Alizarin red S staining. Mineralized nodule formation was significantly increased after PTHrP treatment compared to the GM and the OM groups without PTHrP treatment. The graphs were normalized to GM (^*P < 0.05, ^**P < 0.01, and ^***P < 0.001 compared to GM group; ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 compared to the OM group without PTHrP treatment)