Fig. 5

HPLF migration on P11–4 peptide hydrogel. (a) Cell migration was measured by MTT staining after 4 days, 7 days and 8 days culturing on P11–4 hydrogels (20 mg/ml). b) 3D Z-stack image of migrated HPLF on P11–4 hydrogel after 3 days, taken by confocal microscopy. Scale bar 60 μm. (c) Migration distance (μm) was measured with Axiovision software (Zeiss) from baseline after 4, 7 and 8 days incubation of HPLF on P11–4 (20 mg/ml), n = 3, *p-value ≤0.05, **p-value ≤0.01, *** p-value ≤0.001. (d) Cell migration density in number/cm² after 4 and 8 days incubation of HPDLF. e) Quantification of the maximal migration distance (μm) of HPLF on P11–4 (20 mg/ml) was determined by ImageJ analysis, n = 3, *p-value ≤0.05, **p-value ≤0.01, *** p-value ≤0.001. f) Migration density calculated as cell number/cm² with ImageJ analysis. Pictures proof a high migration capacity of HPLF into fibrillar P11–4 hydrogels