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Fig. 4 | BMC Oral Health

Fig. 4

From: Vinegar inhibits the formation of oral biofilm in situ

Fig. 4

TEM micrographs: gallery of representative pellicle layers after water rinsing and rinsing with vinegar. The blue lines indicated the electron dense layer. “Enamel” indicates the former enamel substrate dissolved during processing of the specimens for TEM analysis. a, c and e Control group, the outer globular layer increased over time, also, the electron-dense basal layer was observed quite thin; b, d and f Vinegar group, the granular structured layer was removed and the subsurface was detected with increasing electron density after 120 min; a and b immediately after rinsing; c and d 30 min after rinsing; e and f 120 min after rinsing. Original magnification: 23,000-fold

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