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Table 1 Primers and probes used in the real-time PCR assays

From: Effects of probiotic bacterium Weissella cibaria CMU on periodontal health and microbiota: a randomised, double-blind, placebo-controlled trial

Bacteria Target genes Primers/Probe sets Amplicon size (bp)
Aggregatibacter actinomycetemcomitans leukotoxin gene 5′-CGGGGCTTTCTACTACGGGA-3′ 123
5′-ATGCCTCAAGCATTCTCGCA-3′
5′-FAM-GGTCAGCTTGGCAATCAGCCC-BHQ1–3’
Campylobacter rectus groEL gene 5′-AAATTTAAGCGGCGACGAGG-3’ 132
5′-TCCTTGCTCACGCTTACGGA-3’
5′-HEX-GGCTTTGACGCGGGCGTAGT-BHQ1–3’
Eikenella corrodens proline iminopeptidase (pip) gene 5′-GCCAACTGCTGCTGGAAGTG-3’ 110
5′-GCCGCTGATTTCGGAGAGTT-3’
5′-HEX- ACAGCCATCGGCACAGGCAT-BHQ1–3’
Fusobacterium nucleatum 16S ribosomal RNA gene 5′-GGCTGTCGTCAGCTCGTGTC-3’ 114
5′-CTCATCGCAGGCAGTATCGC-3’
5′-FAM-AACGAGCGCAACCCCTTTCG-BHQ1–3’
Porphyromonas gingivalis hemagglutinin (phg) gene 5′-ACACGGTGTATCGTGACGGC-3’ 119
5′-GCCGGCTGCGTACTTAACCT-3’
5′-HEX-CGACCTACCGCGATGCAGGA-BHQ1–3’
Prevotella intermedia hemagglutinin (phg) gene 5′-CACACGCTGGCGAAACCTAC-3’ 143
5′-CACGTGGCGTTGCTTCTTTC-3’
5′-HEX-CCGAAGATGCGCCGTTGAAC-BHQ1–3’
Prevotella nigrescens gyrase subunit B (gyrB) gene 5′-AGCAAGCTGTAGGCGAGGCT-3’ 132
5′-GCTGAACACTTTCGCGTGCT-3’
5′-Texas Red-GCTCGTATTGCAGCCCGCAA-BHQ2–3’
Tannerella forsythia karilysin protease gene 5′-TGGCAAATCGCTCATCATCC-3’ 140
5′-TTCCATGTTCCCCAACCACA-3’
5′-Texas Red-CCATTAAGCCCATTGCCCGG-BHQ2–3’
Treponema denticola oligopeptidase B (opdB) gene 5′-AGAAAGGCTTTGGGCGACAG-3’ 127
5′-GCTGGAGCCGTAGCTTCCAT-3’
5′-Cy5-CGGGTCCTCACCCGCTCTTC-BHQ2–3’
Actinomyces viscosus sialidase (nanH) gene 5′-GCTCCCTCATGCTCAACTCG-3’ 140
5′-GATGATCTGGGCGTTGTCCA-3’
5′-Texas Red-GAGCCGGTCCCCGACAAGAA-BHQ2–3’
Enterococcus faecalis gelatinase (gelE)-serine protease (sprE) operon 5′-GATGGCAGTGCGACACCATT-3’ 101
5′-CGATCGTTTTGTTTGCCGAC-3’
5′-HEX-GCTTTTTCCCCGAATGCGGA-BHQ1–3’
Eubacterium nodatum hypothetical protein 5′-TGCTTGCCGGTGACTTAGGA-3’ 130
5′-AAACCGGGCTCAACAACCAT-3’
5′-Texas Red-TTGAGGAGCCGGTGACTTTGG-BHQ2–3’
Group of Streptococcus 16S ribosomal RNA gene 5′-GTACAACGAGTCGCAAGCCG-3’ 149
5′-TACAAGGCCCGGGAACGTAT-3’
5′-FAM-TAATCGCGGATCAGCACGCC-BHQ1–3’
Parvimonas micra 16S ribosomal RNA gene 5′-GAGGAATACCGGTGGCGAAG-3’ 148
5′-GGCACCGAGATTTGACTCCC-3’
5′-FAM-GGTACGAAAGCGTGGGGAGCA-BHQ1–3’
Staphylococcus aureus clumping factor A (clfA) gene 5′-GCGCAAGTAACGAAAGCAAAA-3’ 132
5′-GATTTTGCGCCACACTCGTT-3’
5′-FAM-TGCTGCACCTAAAACAGACGACACA-BHQ1–3’
Streptococcus mutans mannitol-specific enzyme II (mtlA) gene 5′-CAGCGCATTCAACACAAGCA-3’ 103
5′-TGTCCCATCGTTGCTGAACC-3’
5′-HEX-TGCGGTCGTTTTTGCTCATGG-BHQ1–3’