Table 3 Differentially expressed proteins in GCF at T1, T3, and T6 during orthodontic treatment when compared to T0 (pre-treatment). There was a change in the expression of 16 proteins in the GCF. Increased and decreased protein abundance (PA) of significant proteins are represented in the form of ↑ and ↓ (Student’s T-test, p-value ≤0.05)
Protein name | Gene name | Accession number (SwissProt) | pI/MW (kDa) | Sequest score | Peptide matched (% sequence coverage) | Fold change; differential protein abundance (↑/↓) | Student’s t-test; p-value | ||
|---|---|---|---|---|---|---|---|---|---|
T1 | T3 | T6 | |||||||
Angiotensinogen | AGT | P01019 | 6.32/53.12 | 2.21 | 2.47 | 1.03↓ | 1.04↓ | 0.98↓ | 0.03 |
Apolipoprotein D | APOD | P05090 | 5.15/21.26 | 2.11 | 5.82 | 1.20↓ | 1.13↓ | 0.99↓ | 0.05 |
Beta-2-glycoprotein 1 | APOH | P02749 | 7.97/38.27 | 171.74 | 35.36 | 1.18↓ | 1.10↓ | 1.12↓ | 0.02 |
Heat shock 70 kDa protein 1A | HSPA1A | P0DMV8 | 5.66/70.01 | 96.86 | 12.79 | 1.14↓ | 0.99↑ | 1.02↑ | 0.01 |
Hemoglobin subunit beta | HBB | P68871 | 7.28/15.99 | 429.06 | 89.80 | 1.15↓ | 1.14↓ | 1.07↓ | 0.03 |
Histidine-rich glycoprotein | HRG | P04196 | 7.5/59.54 | 12.46 | 2.67 | 1.13↓ | 1.05↓ | 1.18↓ | 0.02 |
Ig heavy variable 4–34 | IGHV4–34 | P06331 | 8.28/16.22 | 16.36 | 10.96 | 1.02↓ | 0.99↑ | 1.01↑ | 0.03 |
Ig kappa variable 3–20 | IGKV3–20 | P01619 | 8.48/11.77 | 187.22 | 31.19 | 1.11↓ | 1.08↓ | 1.01↑ | 0.03 |
Ig heavy variable 3–11 | IGHV3–11 | P01762 | 9.72/13.46 | 19.17 | 15.57 | 1.08↓ | 1.03↓ | 1.12↓ | 0.04 |
Ig heavy constant gamma 1 | IGHG1 | P01857 | 8.19/36.08 | 848.34 | 51.52 | 1.12↓ | 1.07↓ | 0.99↓ | 0.03 |
Ig J chain | IGJ | P01591 | 5.24/18.09 | 15.71 | 16.35 | 0.98↑ | 0.98↑ | 1.05↑ | 0.03 |
Thymidine phosphorylase | TYMP | P19971 | 5.53/50.37 | 14.68 | 2.87 | 1.07↓ | 0.97↑ | 0.83↓ | 0.03 |
Protein S100-A9 | S100A9 | P06702 | 6.13/13.23 | 44.48 | 24.56 | 0.97↑ | 0.95↑ | 1.07↑ | 0.02 |
Thymosin beta-4 | TMSB4X | P62328 | 5.06/5.05 | 143.02 | 54.55 | 1.02↓ | 1.00↓ | 1.04↓ | 0.05 |
Zinc-alpha-2-glycoprotein | AZGP1 | P25311 | 6.05/34.24 | 108.53 | 19.46 | 0.95↑ | 0.90↑ | 1.13↑ | 0.04 |
Vitronectin | VTN | P04004 | 5.8/54.27 | 239.34 | 23.85 | 0.99↑ | 1.00↓ | 1.064↑ | 0.04 |