Fig. 4From: Evaluation of 24 protocols for the production of platelet-rich fibrinReprinted with permission [16]The concentration of cell types in each layer from 1 mL down to the 10th 1-mL sample utilizing the solid-PRF horizontal centrifugation protocol (700g for 8 min). Notice that most of the platelets are evenly distributed throughout the upper plasma layer. Similarly, white blood cells are primarily distributed throughout the upper 4 layers, though not as even as platelets owing to their slightly higher cellular density (Arrows represent the separation between the plasma and red blood cell layer (buffy coat)).Back to article page