Fig. 5

Observed variability of the concentration of cell types in each layer from 1 mL down to the 10th 1-mL sample utilizing the same solid-PRF horizontal centrifugation protocol (700g for 8 min) as depicted in Fig. 4 (owing to difference in patient gender and age). Notice that when compared to Fig. 4, more leukocytes are found within the buffy coat layer. This finding is a result of a patient demonstrating lower hematocrit counts (typically occurring in older female patients), thereby resulting in easier cell-layer separation and more pronounced accumulation within the buffy coat layer (arrows represent the separation between the plasma and red blood cell layer (buffy coat))