Fig. 3

Total protein dosage (n = 12). a Development stage of biofilms at 24 h and 72 h. Single-species biofilms of S. salivarius showed a significant difference at 24 h and 72 h compared to single-species biofilms of C. albicans (p < 0.05). Co-culture biofilms also demonstrated a significant difference between 24 and 72 h (p < 0.05). Connected groups present statistical differences (Mann–Whitney, p < 0.05). Data shown are from box plot: each box contains 50% of the group data; the lower and upper limits represent the 25th and 75th percentiles, respectively; bars represent the minimum and maximum values, and the horizontal line inside the box represents median. b Biofilm types (single-species or co-culture) within the same time interval (24 h or 72 h). Single-species biofilms of S. salivarius and co-culture biofilms at 72 h presented statistically significant differences (p < 0.05). Connected groups present a statistical difference (Mann–Whitney, p < 0.05). Data shown are from the box-plot: each box contains 50% of the group data; the lower and upper limits represent the 25th and 75th percentiles, respectively; bars represent the minimum and maximum values, and the horizontal line inside the box represents median