Table 1 Amplicon sizes (base pairs) results from Multiplex PCR amplification using yeast specific (Universal-UNI1 and UNI2) and corresponding species-specific primers of Candida species
From: Prevalence, virulence and antifungal activity of C. albicans isolated from infected root canals
Species | Primer | Sequence (5′-3′) | Amplicon size (bp) |
|---|---|---|---|
| Â | UNI 1 | Â | Â |
| Â | UNI 2 | Â | Â |
C. albicans | Calb | AGCTGCCGCCAGAGGTCTAA | 583/446 |
C. tropicalis | Ctro | GATTTGCTTAATTGCCCCAC | 583/507 |
C. krusei | Ckru | CTGGCCGAGCGAACTAGACT | 590/169 |
C. glabrata | Cgla | TTGTCTGAGCTCGGAGAGAG | 929/839 |
C. dubliniensis | Cdub | CTCAAACCCCTAGGGTTTGG | 591/217 |
C. parapsilosis | Cpar | GTCAACCGATTATTTAATAG | 570/370 |