Table 1 Lists of primers used in RT-qPCR analysis
Gene name (symbol) | Primers sequence | Product size (bp) | Anneal. temp (°C) |
|---|---|---|---|
Fatty acid-binding protein4 (FABP4) | F: TGAGATTTCCTTCATACTGG R: TGGTTGATTTTCCATCCCAT | 128 | 60 |
Lipoprotein lipase (LPL) | F: AGACACAGCTGAGGACACTT R: GCACCCAACTCTCATACATT | 137 | 60 |
Peroxisome proliferator-activated receptor (PPARγ) | F: TTGCTGTCATTATTCTCAGT R: GAGGACTCAGGGTGGTTCAG | 124 | 60 |
Aggrecan (ACAN) | F: AGTGGATTGGCTTGAACGAC R: AGTGGCGAAGAAGTTGTCAG | 113 | 60 |
Collagen, type X, alpha 1(COL10A1) | F: GCAAACATGCTGCCACAAAC R: GATGAAGAACTGTGCCTTGGTG | 141 | 60 |
Alkaline phosphatases (ALP) | F- AGCTCATCTCCAACATGGAC R- ACCTTGGCTGTAGTCATCTG | 354 | 60 |
Dentin matrix acidic phosphoprotein 1 (DMP-1) | F- AGAGAGAGATGGGAGAGCTGCGC R- TGACCTTCCATCTGCCTCCTGTTC | 128 | 60 |
dentin sialophosphoprotein (DSPP) | F- TCCTTTTGAAGCCTTTTAAGCCATT R- TGGTTTGCTTTGAGGAACTGGAAT | 117 | 60 |
Estrogen receptor alpha (ER-α) | F- TGGGCTTACTGACCAACCTG R- CCTGATCATGGAGGGTCAAA | 115 | 60 |
BCL2-associated X protein (BAX) | F: TGCTTCAGGGTTTCATCCAG R: GGCGGCAATCATCCTCTG | 170 | 60 |
BCL-2 antagonist/killer 1 (BAK) | F: GGCACCTCAACATTGCATGG R: CAGTCTCTTGCCTCCCCAAG | 144 | 60 |
B-cell CLL/lymphoma 2 (BCL2) | F: GGCTGGGATGCCTTTGTG R: CAGCCAGGAGAAATCAAACAGA | 66 | 60 |
Baculoviral inhibitor of apoptosis repeat-containing 5 (BIRC) | F: CAGATGACGACCCCATGCAA R: GAGGCCTCAATCCATGGCAG | 112 | 60 |
Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta (YWHAZ) | F: CGAAGCTGAAGCAGGAGAAG R: TTTGTGGGACAGCATGGATG | 113 | 60 |