Table 1 Lists of primers used in RT-qPCR analysis
| Gene name (symbol) | Primers sequence | Product size (bp) | Anneal. temp (°C) |
|---|---|---|---|
| Fatty acid-binding protein4 (FABP4) |
F: TGAGATTTCCTTCATACTGG R: TGGTTGATTTTCCATCCCAT | 128 | 60 |
| Lipoprotein lipase (LPL) |
F: AGACACAGCTGAGGACACTT R: GCACCCAACTCTCATACATT | 137 | 60 |
| Peroxisome proliferator-activated receptor (PPARγ) |
F: TTGCTGTCATTATTCTCAGT R: GAGGACTCAGGGTGGTTCAG | 124 | 60 |
| Aggrecan (ACAN) |
F: AGTGGATTGGCTTGAACGAC R: AGTGGCGAAGAAGTTGTCAG | 113 | 60 |
| Collagen, type X, alpha 1(COL10A1) |
F: GCAAACATGCTGCCACAAAC R: GATGAAGAACTGTGCCTTGGTG | 141 | 60 |
| Alkaline phosphatases (ALP) |
F- AGCTCATCTCCAACATGGAC R- ACCTTGGCTGTAGTCATCTG | 354 | 60 |
| Dentin matrix acidic phosphoprotein 1 (DMP-1) |
F- AGAGAGAGATGGGAGAGCTGCGC R- TGACCTTCCATCTGCCTCCTGTTC | 128 | 60 |
| dentin sialophosphoprotein (DSPP) |
F- TCCTTTTGAAGCCTTTTAAGCCATT R- TGGTTTGCTTTGAGGAACTGGAAT | 117 | 60 |
| Estrogen receptor alpha (ER-α) |
F- TGGGCTTACTGACCAACCTG R- CCTGATCATGGAGGGTCAAA | 115 | 60 |
| BCL2-associated X protein (BAX) |
F: TGCTTCAGGGTTTCATCCAG R: GGCGGCAATCATCCTCTG | 170 | 60 |
| BCL-2 antagonist/killer 1 (BAK) |
F: GGCACCTCAACATTGCATGG R: CAGTCTCTTGCCTCCCCAAG | 144 | 60 |
| B-cell CLL/lymphoma 2 (BCL2) |
F: GGCTGGGATGCCTTTGTG R: CAGCCAGGAGAAATCAAACAGA | 66 | 60 |
| Baculoviral inhibitor of apoptosis repeat-containing 5 (BIRC) |
F: CAGATGACGACCCCATGCAA R: GAGGCCTCAATCCATGGCAG | 112 | 60 |
| Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta (YWHAZ) |
F: CGAAGCTGAAGCAGGAGAAG R: TTTGTGGGACAGCATGGATG | 113 | 60 |