Fig. 7
From: Melatonin antagonizes lipopolysaccharide-induced pulpal fibroblast responses
Expression of IL- β transcripts of cultured pulpal fibroblasts after incubation with E. coli LPS (20 μ g/mL) and/or various concentrations of exogenous melatonin. Relative densities of the bands from one of 3 independent experiments yielding identical results were shown (7A, 7C). Basal expression of IL- β transcripts was not affected by melatonin co-cultured with pulpal fibroblasts for 3 h (7A, 7B). Co-culture of LPS with melatonin reduced of LPS-induced IL- β expression after 24 h of incubation, particularly 1 mM and 1 μ M melatonin (7C, 7D). Asterisk (*) indicates statistical difference at P-value < 0.05 as determined by One-way ANOVA with Bonferroni’s multiple comparisons