Fig. 2
Viability of apical papilla cells to calcium hydroxide and tricalcium silicate-based cements. a Cartoon showing the methodology. b MTT assay: Graph indicates average and standard error of OD at 570 nm, Photograph of formazan crystal from cells adhered to the top of ProRoot®MTA, BioRoot® RCS, Biodentine™, CaOH2 diluted in PEG and CaOH2 diluted in NaCl 0.9% w/v. Apical papilla cells adhered to a 24 well plate was used as a control. The data are shown as mean ± SD n = 6 Asterisks indicate statistically significant differences. All materials are significant differences to control cells, and between the material significant different between Biodentine™ versus BioRoot® RCS p = 0.0317 and CH diluted in NaCl is significant diffrerent to: ProRoot®MTA (p = 0.0357), Biodentine (p = 0.0357) and CaOH diluted in PEG (p = 0.0095). c Quantification of MTT assay after 72 h of adhesion to, BioRoot® RCS, Biodentine™, Ultracal, CaOH2 diluted in PEG and CaOH2 diluted in NaCl 0.9% w/v. All biomaterial were significantly different to cells adhered to plastic, ProRoot®MTA versus BioRoot® RCS p = 0.0286, Biodentine™ versus BioRoot® RCS p = 0.0286 n = 5. d Table with quantification of pH