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Fig. 9 | BMC Oral Health

Fig. 9

From: A technical note on contamination from PRF tubes containing silica and silicone

Fig. 9

Macroscopic observation of a compressed and fixed A-PRF membrane. a This PRF membrane was divided into seven pieces, designated regions 1 to 7, where region 1 represents the region closest to the red blood cell fraction. b Microscopic observation of A-PRF cross-sections obtained from individual regions. Cross-sections were stained with hematoxylin and eosin (HE). c To confirm morphological similarity, the size of sections was modified to adjust their lengths at similar levels. Arrows represent the direction of gravity force. Summary of platelet distribution under various conditions. Asterisks represent wide-open spaces, and platelets were distributed sparsely. Double asterisks represent wider spaces than single asterisks. Bar scales are 200um. In general, it was found that cells were more evenly distributed at low centrifugation speeds, whereas high-speed centrifugation typically led to more cells accumulated at the back distal surfaces and closer to the buffy coat. Reprinted with permission from Tsujino et al. 2019 [15]

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