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Table 1 Group distribution and experimental analysis

From: An experimental intraradicular biofilm model in the pig for evaluating irrigation techniques

 

microCT

CRP

Experimental groups

    

SEM

qPCR

16S rRNA

Positive control

CNI

EA

UAI

LAI

Pig 1

 + 

 + 

n = 4

    

n = 1

n = 3

n = 1

Pig 2

 + 

 + 

 

n = 4

   

n = 1

n = 3

 

Pig 3

 + 

 + 

  

n = 4

  

n = 1

n = 3

 

Pig 4

     

n = 4

 

n = 1

n = 3

 

Pig 5

      

n = 4

n = 1

n = 3

 
  1. Twenty roots from 10 lower deciduous mandibular second premolars were analyzed from 5 pigs. Periapical bone defects and the inflammation stage were confirmed by micro CT and CRP in 3 animals. For irrigation comparisons, 4 canals (n = 4) were obtained from the lower deciduous mandibular second premolar of each pig. One distal root from each group was observed by SEM. The remaining three roots were used for qPCR. 16S rRNA was analyzed from one root of the positive control group. CRP: C-reactive protein assay; CNI: Conventional needle irrigation; EA: EndoActivator; UAI: Ultrasonically activated irrigation; LAI: Laser-activated irrigation