Table 1 Primers used in qPCR
From: Analysis of oral microbial dysbiosis associated with early childhood caries
Forward primer | Reverse primer | Amplicon size (bp) | |
|---|---|---|---|
Total bacteria | 5′-TCCTACGGGAGGCAGCAGT -3′ | 5′-GGACTACCAGGGTATCTAATCCTGTT-3′ | 466 |
S. mutans | 5′-GCCTACAGCTCAGAGATGCTATTCT-3′ | 5′-GCCATACACCACTCATGAATTGA-3′ | 98 |
Lactobacillus spp. | 5′-TGGAAACAGGTGCTAATACCG-3′ | 5′-GTCCATTGTGGAAGATTCCC-3′ | 231 |
S. Sobrinus | 5′-TTCAAAGCCAAGACCAAGCTAGT-3′ | 5′-CCAGCCTGAGATTCAGCTTGT-3′ | 88 |
ldh gene | 5′-CTTGATACTGCTCGTTTCCGTC-3′ | 5′-GAGTCACCATGTTCACCCAT-3′ | 93 |
F-ATPase gene | 5′-CGGATGCGTGTTGCTCTTACTG-3′ | 5-GGCTGATAACCAACGGCTGATG-3′ | 167 |