Fig. 6
From: Human dental pulp stem cell responses to different dental pulp capping materials
Effect of ProRoot® MTA and Biodentine™ on odonto/osteogenic differentiation was evaluated. a ALP activity at day 10 after osteogenic induction and mineralization was examined using Von Kossa staining and alizarin red s staining at day 14. Scale bars indicate 40 μm. b The relative absorbance of the eluted alizarin red s dye. c The mRNA expression of odonto/osteogenic differentiation markers (RUNX2, DMP1, DSPP, and OCN) was examined using real-time polymerase chain reaction at day 4 and 10. Bars indicate a significant difference (Mann Whitney U test, p < 0.05)