Material type | Description |
---|---|
Media preparation for MC3T3-E1 cells | Alpha modified Eagle’s medium (α-MEM) (Invitrogen, USA) supplemented with 1 mM sodium pyruvate, 10% (v/v) fetal bovine serum (FBS) (Biowest, South America) and 1% (v/v) penicillin/streptomycin (Invitrogen, USA) were used as a complete media |
Media preparation for DPSC and SHED | Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen, USA) supplemented with 1% Glutamax, 10% (v/v) fetal bovine serum (FBS) (Biowest, South America) and 1% (v/v) penicillin/streptomycin (Invitrogen, USA) were used as a complete media |