Table 3 The concentration of alkaline phosphatase analysis components

5 mM MgSO₄

12.3 mg of MgSO₄ + 10 ml ddi

(from this mixture, 40 µl for each well)

Bicarbonate-carbonate buffer

Na₂CO₃ + NaHCO₃ (PH 10)

Na₂CO₃ = 317.97 mg + 15 ml ddi

NaHCO₃ = 105.01 mg + 15 ml ddi

10.5 ml of Na₂CO₃ + 4.5 ml of NaHCO₃ (from this mixture, 40 µl for each well plate)

P-nitrophenyl phosphate (PNP-P)

2.782 mg + 2 ml ddi (from this mixture, 10 µl for each well plate)

Sodium hydroxide (NaOH)

1.6 mg of NaOH + 20 ml ddi ( from this mixture, 100 µl for each well plate)