Fig. 1

Descriptive analysis. Boxplot shows the data distribution of Failed, Unknown, Predicted, Alpha and Rarefaction variables, comparing (a) Amplicon sequencing and (b) Shotgun metagenomics approaches. Normality analysis was performed using the Shapiro Wilk test and the Mann–Whitney U test evaluated the differences of the variables between the sequencing approaches. All variables showed a significant difference (p < 0.05) between Amplicon sequencing and Shotgun metagenomics, except Predicted. Note that Failed did not present any data in the Amplicon sequencing