Table 1 RT-qPCR primer sequences for reference genes (TBP, RPL22, PPIB) and target genes
Gene symbol | Gene name | 5′-forward primer-3′ | 5′-reverse primer-3′ |
|---|---|---|---|
PPIB | Peptidylprolyl isomerase A | TTCCATCGTGTAATCAAGGACTTC | GCTCACCGTAGATGCTCTTTC |
RPL22 | Ribosomal protein L22 | TGATTGCACCCACCCTGTAG | GGTTCCCAGCTTTTCCGTTC |
IL-6 | Interleukin-6 | TGGCAGAAAACAACCTGAACC | CCTCAAACTCCAAAAGACCAGTG |
PTGS-2 | Prostaglandin-endoperoxide synthase-2 | GAGCAGGCAGATGAAATACCAGTC | TGTCACCATAGAGTGCTTCCAAC |
TBP | TATA binding protein | CGGCTGTTTAACTTCGCTTCC | TGGGTTATCTTCACACGCCAAG |
TNF | Tumor necrose factor | GAGGCCAAGCCCTGGTATG | CGGGCCGATTGATCTCAGC |
OPG | Osteoprotegerin | TGTCTTTGGTCTCCTGCTAACTC | ACGCTCCAGGACTTATACCG |
RANKL | Receptor activator of NFkB ligand | ATACCCTGATGAAAGGAGGA | GGGGCTCAATCTATATCTCG |
NOX-4 | NADPH oxidase 4 | TCTGCCTGTTCATCTGGCTCTCCA | AGCCAAGAGTGTTCGGCACATGGGTA |