Fig. 1
From: Effects of iRoot SP on osteogenic differentiation of human stem cells from apical papilla
Isolation, culture, and characterization of human stem cells of the apical papilla (hSCAPs). A Primary cultured hSCAPs at 7Â days (P0). The 3rd passage hSCAPs exhibit spindle-like morphology (P3). B Osteogenic differentiation of hSCAPs: hSCAPs were cultured with or without osteogenic-induction factors for 3Â weeks, the mineralized nodules formed are positive to Alizarin red staining. C Flow cytometry analysis of hSCAP surface markers. hSCAPs test positive the for mesenchymal stem cell markers CD24 and CD146 and the negative expression hematopoietic stem cell marker CD45. D Characterization of hSCAPs by immunofluorescence staining: positive for vimentin, STRO-1, and CD24, but not for keratin