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Fig. 5 | BMC Oral Health

Fig. 5

From: B cell activating factor regulates periodontitis development by suppressing inflammatory responses in macrophages

Fig. 5

BAFF knockdown inhibited the LPS-induced inflammatory response of macrophages and promoted the macrophage phenotype switch from M1 to M2. RAW 264.7 cells were treated with or without BAFF siRNA for 24 h. a siRNA knockdown of BAFF is confirmed by real-time PCR. b Real-time PCR was used to measure the level of iNOS by applying different concentrations of P. gingivalis LPS (0 ng/mL, 1 ng/mL, 10 ng/mL, 100 ng/mL, 1000 ng /mL) in RAW 264.7 cells for 24 h. The level of iNOS was upregulated by applying of 100 ng/mL P. gingivalis LPS in RAW 264.7 cells. c, d Real-time PCR analysis of the gene expression of the M1-related TNF-α and the M2-related IL-10. e, f Concentrations of TNF-α and IL-10 in the cell supernatant measured by ELISA. Data are shown as the mean ± SD of three independent experiments. NS, the difference was not significant,*P < 0.05, **P < 0.01 and ***P < 0.001 using one-way ANOVA with Tukey's test

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