Fig. 3
From: Effect of epigallocatechin gallate on dental biofilm of Streptococcus mutans: An in vitro study
Confocal laser scanning microscopy (CLSM) images of SYTO 9/propidium iodide (PI)-stained biofilms. Epigallocatechin gallate (EGCG) reduced the number of live bacteria and the amount of exopolysaccharides (EPS) in a dose-dependent manner. Computerized 3D reconstruction of the biofilm layers after treating S. mutans with different concentrations of EGCG for 24 h, as recorded by CLSM and generated by the Nikon Imaging Software (NIS-elements). a Control. b–d Increasing concentrations of EGCG (mg/ml). A representative sample of each treatment is shown. Green color represents live cells, red color represents dead cells and blue color represents the EPS