Fig. 4

Quantification of the SYTO 9/propidium iodide (PI) staining of biofilms formed in the absence or presence of epigallocatechin gallate (EGCG). Quantification of the fluorescence intensity of each biofilm layer after treating S. mutans with increasing concentrations of EGCG for 24 h. A Live bacteria stained by SYTO 9. B Dead bacteria stained by PI. C Exopolysaccharides (EPS) production stained by Alexa Fluor⁶⁴⁷-conjugated ConA