Fig. 5

si-Runx2 plus miR-30a-5p inhibitors combatted the efficiency of osteogenesis and inflammation from si-Runx2. A The expression of Runx2 was examined by qPCR in MC3T3-E1 cells after 24 h after transfected with three si-Runx2 (si-Runx2-459, si-Runx2-909, si-Runx2-1459) and relative controls (si-NC). B The expression of Runx2 was examined by qPCR in MC3T3-E1 cells after 24 h after transfected with si-Runx2, relative controls (si-NC) and si-Runx2 + miR-30a inhibitors. C The expression of ALP, COL-1 and OC were examined by qPCR in MC3T3-E1 cells after 24 h after transfected with si-Runx2, relative controls (si-NC) and si-Runx2 + miR-30a inhibitors. D The expression of IL-1-R1, IL1-R2 and TNFR were examined by qPCR in MC3T3-E1 cells after 24 h after transfected with si-Runx2, relative controls (si-NC) and si-Runx2 + miR-30a inhibitors. E The level of ALP was examined by ALP staining in MC3T3-E1 cells after 7 days after transfected with si-Runx2, relative controls (si-NC) and si-Runx2 + miR-30a inhibitors. F The level of Alizarin red was examined by Alizarin red staining in MC3T3-E1 cells after 21 days after transfected with si-Runx2, relative controls (si-NC) and si-Runx2 + miR-30a inhibitors. All data are shown as mean ± SEM. *p < 0.05; **p < 0.01